MiSeq Resources
System Literature
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16S Metagenomics Studies with the MiSeq SystemApplication note | PDF | < 1 MB
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MiSeq BrochureBrochure | PDF | 3 MB
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MiSeq System applications,MiSeq Applications BrochureBrochure | PDF | 3 MB
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E. coli Sequencing on the MiSeq System and Ion Torrent PGM SystemApplication note | PDF | < 1 MB
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High-Speed, Multiplexed 16S Microbial Sequencing on the MiSeq SystemApplication note | PDF | < 1 MB
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Nextera Sample Prep for the MiSeq SystemApplication note | PDF | < 1 MB
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Sequencing Library QC on the MiSeq SystemApplication note | PDF | < 1 MB
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Sequencing the Rapidly Evolving Influenza A Virus on the MiSeq SystemApplication note | PDF | < 1 MB
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De novo Bacterial Sequencing on the MiSeq SystemTechnical note | PDF | < 1 MB
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Optimizing Cluster Density on Illumina Sequencing SystemsTechnical note | PDF | 3 MB
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Faster Access to Genomic Informationcase_study | PDF | < 1 MB
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Illumina Proactive Service connectivity guide - MiSeqBrochure | PDF | < 1 MB
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Pan-Viral Sequencing on Illumina Benchtop Systems Application NoteApplication note | HTML |
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Microbial de novo assembly with linked-read technologyApplication note | PDF | 2 MB
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Application note | PDF | 2 versions
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Data sheet | PDF | 9 versions
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Brochure | PDF | 3 versions
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Technical note | PDF | 11 versions
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Technical note | PDF | 9 versions
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Illumina DNA PrepData sheet | PDF | < 1 MB
Product Selection and Experiment Planning Tools
Find tools to help you choose the right products for your needs and plan your experiments. Identify the right library prep kit or microarray, calculate sequencing coverage, explore methods compiled from publications, design custom assays, and more.
Explore Tools
Education and Support Resources
NEW MiSeq Self-Service Resources
Access the MiSeq System interactive guide for general use, troubleshooting, and other additional resources.
MiSeq System Guide
Information about instrument components, software, required products, performing a sequencing run, and instrument maintenance.
MiSeq Site Prep Guide
Find lab specifications and requirements for preparing your lab for a MiSeq System.
Sequencing Coverage
Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.
Quality Scores
Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.
MiSeq Documentation
Find system guides, related application and technical notes, and other supporting documentation for the MiSeq System.
Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.
Videos and Webinars
Using the MiSeq for Microbial Sequencing Applications
Practical information for new MiSeq users with many tips, tricks, and microbial examples gleaned from long instrument experience.
Investigating Tree Sloths with Mixed Amplicon Sequencing
Researchers used paired-end 16S amplicon sequencing on the MiSeq System to explore their microbe-cycling hypothesis.
Ebola and Sexual Transmission
Researchers compared viral RNA sequences from samples, and found substitutions absent from other Western African Ebola genomes.