Featured Publications
Clonal evolution and resistance to EGFR blockade in the blood of colorectal cancer patients.
Nat Med 2015; 21(7):795-801
Pancreatic β cell enhancers regulate rhythmic transcription of genes controlling insulin secretion.
Science 2015; 350(6261):aac4250
An oncogenic MYB feedback loop drives alternate cell fates in adenoid cystic carcinoma.
Nat Genet. 2016; 48:265-72
NextSeq 550 System Resources
System Literature
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Brochure | PDF | 3 versions
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Direct Bacterial Colony Sequencing with the Nextera™ DNA Flex Library Preparation KitApplication note | PDF | 1 MB
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Laboratory Experience Using Illumina Next-Generation Sequencing for Noninvasive Prenatal Testing Application NoteApplication note | PDF | < 1 MB
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Application note | PDF | 2 versions
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NextSeq 550 RNA-Seq solutionApplication note | PDF | < 1 MB
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NextSeq 550 amplicon sequencing solutionApplication note | PDF | < 1 MB
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NextSeq 550 exome sequencing solutionApplication note | PDF | < 1 MB
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QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 SystemApplication note | PDF | 2 MB
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NextSeq System Suite Installer Version 4.0 UpgradeTechnical note | HTML |
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Technical note | PDF | 2 versions
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Unify single-cell gene expression and chromatin accessibilityTechnical note | PDF | 1 MB
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Illumina CMOS Chip and One-Channel SBS Chemistrytechnology_spotlights | PDF | < 1 MB
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Direct bacterial colony sequencing with Illumina DNA PrepApplication note | PDF | < 1 MB
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Illumina DNA Prep crude lysate protocol for NGSApplication note | PDF | < 1 MB
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Technical note | PDF | 11 versions
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Explore the transcriptome with single-cell resolutionTechnical note | PDF | 1 MB
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Multiomic interrogation of the immune system at single-cell resolutionTechnical note | PDF | 1 MB
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Technical note | PDF | 9 versions
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Illumina DNA PrepData sheet | PDF | < 1 MB
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Data sheet | PDF | 5 versions
Product Selection and Experiment Planning Tools
Find tools to help you choose the right products for your needs and plan your experiments. Identify the right library prep kit or microarray, calculate sequencing coverage, explore methods compiled from publications, design custom assays, and more.
Explore Tools
Education and Support Resources
NextSeq 550 Manuals and Documents
Find the system guide, site prep guide, technical notes, and other supporting documentation.
Instrument Buying Options
Don’t let your budget hold you back. Explore different buying options to find which best fits your needs.
Sequencing Online Training
Online Illumina sequencing courses are free, interactive, and available any time.
Personalized Training Workflow Selection Tool
This tool will help you determine the best hands-on sequencing training for your needs based on your area of interest, application, library preparation kit, and platform.
Quality Scores
Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.
Sequencing Coverage
Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.
Sequencing Troubleshooting Tips
These short videos provide expert tips for common sequencing issues such as overclustering and inconsistent quantitation.
Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.
Customer Stories
Deeper Insights on RNA Sequencing
RNA sequencing on the NextSeq 550 System provides deeper insights for this lab’s cutting-edge work in virology, moving beyond just gene expression.
View VideoBringing Bioinformatics Pipeline In-House Reduces Costs and Decreases Turnaround Time
Phosphorus uses the DRAGEN Bio-IT Platform to perform genomics data analysis onsite and at an accessible price point.
Read articleDeciphering the Role of Long Non-Coding RNA in Cancer
Researchers are using RNA-Seq to reveal how lncRNAs could be used to identify, measure, and treat cancer.
Read article