The HiSeq 3000/HiSeq 4000 Systems have been discontinued. We will continue to provide full support of the instruments and supply the reagents through February 28, 2023 (for the HiSeq 3000 System) or March 31, 2024 (for the HiSeq 4000 System). To find an alternative instrument, we recommend using the Sequencing Platform Comparison Tool.
HiSeq 3000/HiSeq 4000 Products and Services
Featured Products
HiSeq 3000/4000 SBS Kit
HiSeq 3000/4000 SBS Kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, delivering extremely high cluster densities and significant increases in data output and daily throughput.
Learn MoreHiSeq X Reagent Kits
Kits include SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
MiSeq Reagent Kit v3
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
TruSeq Stranded mRNA
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
Automation Solutions
High Thoughput Library Prep Automation
Illumina offers high throughput-compatible library prep kits and partnerships with leading automation vendors.
BaseSpace Clarity LIMS
Our laboratory information management system helps researchers track samples and optimize procedures and workflows.
Software and Informatics Solutions
HiSeq 3000/HiSeq 4000 System Control Software
User-friendly touchscreen–operated instrument control software guides users through each step of the sequencing workflow, from experiment setup to reagent and flow cell loading. The software also generates real-time quality statistics for accurate run monitoring.
View SoftwareBaseSpace Sequence Hub | Run management and simplified bioinformatics
Easy-to-use cloud-based genomic run management and bioinformatics compute and storage environment for secure, high-performing sequencing operations.
BaseSpace Variant Interpreter
BaseSpace Variant Interpreter allows scientists to rapidly identify biologically significant variants from human genomic data.
Instrument Services
Comprehensive Scientific Support
Illumina services and support begins when the HiSeq Series is delivered. Illumina scientists and engineers assist with NGS system installation and setup and train laboratory personnel. In addition to onsite support, training courses are available to bring laboratory personnel quickly up to speed.
Illumina scientists are available 24/7 globally to answer questions every step of the way, enabling researchers to focus on making the next breakthrough discovery.
Illumina Product Support Services
In addition to the 1-year basic service warranty included with system purchase, Illumina offers maintenance, repair, and qualification solutions.
Illumina Training
Get high-quality results on Illumina technology even faster with instructor-led or hands-on courses and web-based training options.
Instrument Qualification Services
Verify instrument installation, operation, or performance and obtain an audit-ready report to help meet regulatory requirements.
Proactive Instrument Monitoring
Connect to Illumina Proactive to access this complimentary instrument performance monitoring and proactive support service.